Applied Methods and Devices

Methods

    Analytical
    • High-performance liquid chromatography
    • Gas chromatography
    • Ion chromatography
    • FIA (HPLC: OPA-NAC)
    • (Fluorescence) Spectrophotometry
    Molecular biological
    • qPCR and digital PCR (dPCR)
    • 16S rRNA sequencing including library preparations and bioinformatic analyses
    • Whole genome sequencing including bioinformatic analyses
    • Nanopore long-read sequencing
    Microbiological
    • aerobic and anaerobic cultivation
    • semi-continuous anaerobic digestion
    • batch AD
    • handling of anaerobic pure and co cultures
    • culture separation under anaerobic conditions

      Devices

      Analytical
      • Shimadzu GC-2010 gaschromatograph (Shimadzu, Japan) with FI and TC detectors
      • Shimadzu Prominence HPLC Systems (modular) with UV-Vis-, Refractive Index (RI)-, Fluorescence (RF-20Axs)- and Photodiode Array detectors (SPD), and pumps for post-column derivatisation (Shimadzu, Japan)
      • Hitachi U2001 UV-VIS Spectrophotometer (Hitachi, Japan)
      • Ion Chromatography Systems (Metrohm, Switzerland)
      • Hitachi F-4500 fluorescence spectrophotometer (Hitachi, Japan)
      • FTIR-8201PC Spectrophotometer (Shimadzu, Japan)
      Molecular biological
      • qPCR device Rotor-Gene Q (Qiagen, Denmark)
      • digitalPCR System QIAcuity One, 5plex (Qiagen, Denmark)
      • QIAxcel instrument for capillary electrophoresis (Qiagen, Denmark)
      • Biomek liquid handler (Coulter Beckman, USA) with various equipment for automatic pipetting and dilutions, extraction, NGS library preparation, application of magnetic beads etc.
      • Fast-Prep 24 (MP Biomedicals, USA) for cell-disruption (bead beating) during DNA/RNA extraction
      • Nanopore MinION long-sequencing device (Oxford Nanopore Technologies, UK)
      Microbiological
      • Climatic chambers and anaerobic jars
      • Glove boxes for working under oxygen-free conditions
      • Automatic gas exchanger and pressure filling device (GR-Instruments, Netherlands) for preparing anoxic media
      • Various Widdle-flasks
      • Gas-tight batch reactors
      • 0.5 – 12 L bioreactors (Sartorius or self-made): automatic temperature control, automatic dosing/feeding/deduction system (time-controlled multi-channel pump), on-line biogas counting (AMPTS), stirring system
      • AMPTS II Automatic Methane Potential Test System (BPC Instruments, Sweden)
      • Research microscope including fluorescence equipment (NIKON eclipse) with high resolution imaging option and evaluation software
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