Dora Čertnerová
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Guest scientist, PostDoc |
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Employment History
since June 2023: Postdoctoral researcher at the Research Department for Limnology, Mondsee, Austria
2021-2022: Maternity leave
2015-2022: PhD - Charles University, Faculty of Science, Department of Botany, Thesis: Genome size variation in microalgae and its evolutionary consequences; defended 27/09/2022 (supervisor: doc. Mgr. Pavel Škaloud, Ph.D.)
since 2017: Postgraduate researcher at Charles University, Faculty of Science, Department of Botany
2014-2017: Molecular lab technician at Charles University, Faculty of Science
2013-2015: MSc - Charles University, Faculty of Science, Department of Botany, Thesis: Reconstructing the evolution and phylogenetic relationships of silica-scaled chrysophyte genus Mallomonas (supervisor: doc. Mgr. Pavel Škaloud, Ph.D.)
2010-2013: BSc - Charles University, Faculty of Science, Department of Botany, Thesis: The significance of silica structures in chromist microorganisms (supervisor: Mgr. Pavel Škaloud, Ph.D.)
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Research interests
My research field is genome size variation and its evolutionary consequences, algal DNA flow-cytometry and synthetic polyploid induction.
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Projects and funding
- 2023-2025 – Czech Science Foundation (Postdoc Individual Fellowship Outgoing, 23-05546O): Shaping the genome: How environmental factors affect the DNA content evolution in populations of planktonic algae?
- 2023 - Martina Roeselová Memorial Fellowship supporting PhD students and postdocs conducting competitive research who are also the primary caregiver of a preschool child
- 2017-2019 - Charles University Student Grant Agency. The population-genetic structure of protists with Synura petersenii as a model species
- 2018 - Scholarship supporting participation in Workshop on Genomics 2018, Český Krumlov
- 2018 - Mobility fund of Charles University
- 2018 - Holz-Conner Travel Award, International Society of Protistologists
- 2017 - Erasmus+ Traineeship
- 2016 - Paul C. Silva Student Grant. International Phycological Society
Fellowships
- 2023-2024: Postdoc Individual Fellowship at Innsbruck University, Mondsee, Austria (mentor: Dr. Claus-Peter Stelzer) - Shaping the genome: How environmental factors affect the DNA content evolution in populations of planktonic algae? [Methods: Flow cytometry, Genome Assembly]
- 2018: 6-month fellowship at University of Guelph, Canada (mentor: Prof. T. Ryan Gregory) – Genome size analysis of various algal samples [Methods: Flow cytometry, Feulgen image analysis densitometry]
- 2017: 3-month fellowship at Lund University, Sweden (mentor: Prof. Karin Rengefors) - Population genetic structure of microalga Gonyostomum semen [Methods: RAD sequencing]
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Publications
2024
Kuhl R., Strassert J.F.H., Čertnerova D., Varga E., Kreuz E., Lamatsch D.K., Wuertz S., Köhler J., Monaghan M.T., Stöck M. (2024). The haplotype-resolved Prymnesium parvum (type B) microalga genome reveals the genetic basis of its fish-killing toxins, Current Biology 34: 1-9, https://doi.org/10.1016/j.cub.2024.06.033 [Add to Citavi project by DOI]
Kuhl R., Strassert J. F., Čertnerova D., Varga E., Kreuz E., Lamatsch D. K., Wuertz S., Köhler J., Monaghan M.T., Stöck M. (2024). The haplotype-resolved Prymnesium parvum (type B) microalga genome reveals the genetic basis of its fish-killing toxins. bioRxiv, 2024-03.
In total, I (co)-authored 12 peer-reviewed publications, 7 as the first author (including 2 single-author publications)
Total. no. of citations: 136, H-index: 7
Čertnerová D., Čertner M., Škaloud, P. (2022). Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle , Algal Research 64: 102707, https://doi.org/10.1016/j.algal.2022.102707
Čertnerová D. (2022). Meet the challenges of analyzing small genomes using flow cytometry (commentary) – Cytometry Part A, 101: 707-709, https://doi.org/10.1002/cyto.a.24485
Čertnerová D., Čertner M., Škaloud P. (2022). Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle. Algal Research 64: 102707, https://doi.org/10.1016/j.algal.2022.102707
Čertnerová, D. (2021). Nuclei isolation protocols for flow cytometry allowing nuclear DNA content estimation in problematic microalgal groups, Journal of Applied Phycology 33: 2057–2067, https://doi.org/10.1007/s10811-021-02433-z
Čertnerová D., Galbraith, D. (2021). Best practices in the flow cytometry of microalgae – Cytometry Part A, 99: 359-364 https://doi.org/10.1002/cyto.a.24328
Galbraith D., Loureiro J., Antoniadi I., Bainard J., Bureš P., Cápal P., Castro M., Castro S., Čertner M., Čertnerová D., Chumová Z., Doležel J., Giorgi D., Husband B. C., Kolář F., Koutecký P., Kron P., Leitch I. J., Ljung K., Lopes S., Lučanová M., Lucretti S., Ma W., Melzer S., Molnár I., Novák O., Poulton N., Skalický V., Sliwinska E., Šmarda P., Smith T. W., Sun G., Talhinhas P., Tárnok A., Temsch E. M., Trávníček P., Urfus, T. (2021). Best practices in plant cytometry – Cytometry 99: 311–317, https://doi.org/10.1002/cyto.a.24295
Rengefors K., Gollnisch R., Sassenhagen I., Aloisi K.H., Svensson M., Lebret K., Čertnerová D., Cresko W.A., Bassham S., Ahrén D. (2021). Genome-wide single nucleotide polymorphism markers reveal population structure and dispersal direction of an expanding nuisance algal bloom species, Molecular Ecology 30: 912–925, https://doi.org/10.1111/mec.15787
Čertnerová, D., Škaloud, P. (2020). Substantial intraspecific genome size variation in golden-brown algae and its phenotypic consequences. - Annals of Botany, 126: 1077–1087 [IF = 4.357]. https://doi.org/10.1093/aob/mcaa133
Čertnerová, D., Čertner, M., Škaloud, P. (2019). Molecular phylogeny and evolution of phenotype in silica-scaled chrysophyte genus Mallomonas, Journal of Phycology 55: 912–923, https://doi.org/10.1111/jpy.12882
Gusev S. E., Čertnerová D., Škaloudová M., Škaloud P. (2018). Exploring Cryptic Diversity and Distribution Patterns in the Mallomonas kalinae/rasilis Species Complex with a Description of a New Taxon – Mallomonas furtiva sp. nov., Journal of Eukaryotic Microbiology 65: 38-47, https://doi.org/10.1111/jeu.12427
Pusztai M., Čertnerová D., Škaloudová M., Škaloud P. (2016). Elucidating the phylogeny and taxonomic position of the genus Chrysodidymus Prowse (Chrysophyceae, Synurales), Cryptogamie, Algologie, 37: 297-307, https://doi.org/10.7872/crya/v37.iss4.2016.297
Hanzl M., Kolář F., Nováková D., Suda, J. (2014). Nonadaptive processes governing early stages of polyploid evolution: Insights from a primary contact zone of relict serpentine Knautia arvensis (Caprifoliaceae), American Journal of Botany 101(6): 935-945, https://doi.org/10.3732/ajb.1400005
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Supervision of students
- since 2021 - master student N. Čížková (thesis: Evolution of nuclear DNA content variation in the genus Mallomonas and its ecophysiological consequences)
- since 2020 – advisor of master student J. Lukeš (thesis: Evolution of genome size in streptophyte algae genus Zygnema and its ecological consequences)
- 2020-2021 - bachelor student N. Čížková (thesis: Evolution of silica structures in SAR protists)
- 2019-2020 - bachelor student J. Lukeš (thesis: Genome size estimation in protists)
- February 2020 - four high school students from French Agriculture High school of Blanquefort
Teaching
Lecturer (2015-) of BSc. practical courses at the Charles University, Faculty of Science
- Field course in botany, Non-vascular plant botany, Plant morphology, Protistology
Science popularization, academic activities, awards
- 2019 - Bohuslav Fott Prize for the best student publication (Czech Phycological Society)
- 2016 - student member of the academic senate at the Charles University, Faculty of Science
- 2014-2021 - guiding public tours of the Botanical Garden of the Faculty of Science, Charles University (in Czech and in English)
- 2014-2016, 2019 - introducing algal groups to the public at Fascination of Plants Day.
Reviewing experience
- reviewer of articles for Nova Hedwigia, Cytometry Part A, and Diversity
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