Publications

2024

R. Bereiter #, L. Flemmich #, K. Nykiel #, S. Heel, S. Geley, M. Hanisch, C. Eichler, K. Breuker, A. Lusser*, R. Micura*; 'Engineering covalent small molecule–RNA complexes in living cells'; Nat. Chem. Biol. 2024, accepted. # these authors contributed equally
L. Flemmich, R. Bereiter, R. Micura*; 'Chemical synthesis of modified RNA'; Angew. Chem. Int. Ed. 2024, 63, e202403063.
K. Bartosik, R. Micura*; 'Access to capped RNA by chemical ligation'; RSC. Chem. Biol. 2024, 5, 1104-1110.
J. Thaler, C. Mitteregger, L. Flemmich, R. Micura*; 'A Universal Support for the Solid-Phase Synthesis of Peptidyl-tRNA Mimics'; ChemBioChem 2024, online first.
H. Bernhard, H. Petržílková, B. Popelářová, K. Ziemkiewicz, K. Bartosik, M. Warmiński, L. Tengo, H. Gröger, L. G. Dolce, Cameron D. Mackereth, R Micura, J. Jemielity, E. Kowalinski*; 'Structural basis of Spliced Leader RNA recognition by the Trypanosoma brucei cap-binding complex'; Nat. Commun., accepted.
S. Heel, F. Juen, K. Bartosik, R. Micura, C. Kreutz, K. Breuker*; 'Resolving the intricate binding of neomycin B to multiple binding motifs of a neomycin-sensing riboswitch aptamer by native top-down mass spectrometry and NMR spectroscopy'; Nucleic Acids Res. 2024, 52, 4691-4701.
M. Kompatscher, K. Bartosik, K. Erharter, R. Plangger, F. S. Juen, C. Kreutz, R. Micura, E. Westhof, M. D. Erlacher*. Contribution of tRNA sequence and modifications to the decoding preferences of E. coli and M. mycoides tRNAGlyUCC for synonymous glycine codons. Nucleic Acids Res. 2024, 52, 1374-1386.

2023

Y. S. Polikanov*, M. Etheve-Quelquejeu, R. Micura*; 'Synthesis of peptidyl-tRNA mimics for structural biology applications'; Acc. Chem. Res. 2023, 56, 2713-2725.
C. Eichler #, M. Himmelstoß #, R. Plangger, L. I. Weber, M. Hartl, C. Kreutz*, R. Micura*; 'Advances in RNA labeling with trifluoromethyl groups'; Chem. Eur. J. 2023, 29, e202302220. # these authors contributed equally
J. Thaler #, E. Syroegin #, K. Breuker, Y. Polikanov*, R. Micura*; 'Practical synthesis of N-formylmethionylated peptidyl-tRNA mimics'; ACS Chem. Biol. 2023, 18, 2233–2239. # these authors contributed equally
S. Heel, K. Bartosik, F. Juen, C. Kreutz, R. Micura, K. Breuker*; 'Native top-down mass spectrometry uncovers two distinct binding motifs of a functional neomycin-sensing riboswitch aptamer'; J. Am. Chem. Soc. 2023, 145, 15284–15294.
X. Xu #, M. Egger #, C. Li #, H. Chen, R. Micura*, A. Ren*; 'Structure-based investigations of the NAD+-II riboswitch'; Nucleic Acids Res. 2023, 51, 54–67. # these authors contributed equally

2022

M. Egger, R. Bereiter, S. Mair, R. Micura*; 'Scaling catalytic contributions of small self-cleaving ribozymes'; Angew. Chem. Int. Ed. 2022, 61, e202207590.
E. A. Syroegin #, L. Flemmich #, D. Klepacki, N. Vazquez-Laslop, R. Micura*, Y. Polikanov*; 'Structural basis for the context-specific action of the classic peptidyl transferase inhibitor chloramphenicol'; Nat. Struct. Mol. Biol. 2022, 29, 152–161. # these authors contributed equally; see News&Views by C. Crowe-McAuliffe & D. N. Wilson, Putting the antibiotics chloramphenicol and linezolid into context, Nat. Struct. Mol. Biol. 2022, 29, 79–91.
S. Moreno, M. Brunner, I. Delazer, D. Rieder, A. Lusser,* R. Micura*; 'Synthesis of 4-thiouridines with prodrug functionalization for RNA metabolic labeling'; RSC Chem. Biol. 2022, 3, 447–455.
S. Moreno, J. M. Ramos Pittol, M. Hartl, R. Micura*; 'Robust synthesis of 2'-azido modified RNA from 2'-amino precursors by diazotransfer reaction'; Org. Biomol. Chem. 2022, 20, 7845–7850.
S. Moreno, M. Fickl, I. Bauer, M. B., A. Rázková, D. Rieder, I. Delazer, R. Micura,* A. Lusser*; 6-Thioguanosine monophosphate prodrugs display enhanced performance against thiopurine-resistant leukemia and breast cancer cells, J. Med. Chem. 2022, 65, 15165–15173.
S. Mair, K. Erharter, E. Renard, K. Brillet, M. Brunner, A. Lusser, C. Kreutz*, E. Ennifar,* R. Micura*; 'Towards a comprehensive understanding of RNA deamination – Synthesis and properties of xanthosine-modified RNA'; Nucleic Acids Res. 2022, 50, 6038–6051.
R. Bereiter, E. Renard, K. Breuker, C. Kreutz, E. Ennifar, R. Micura*; '1-Deazaguanosine modified RNA – The missing piece for functional RNA atomic mutagenesis'; J. Am. Chem. Soc. 2022, 144, 10344–10352.
R. Bereiter, M. Oberlechner, R. Micura*; 'A new route for the synthesis of 1-deazaguanine and 1-deazahypoxanthine'; Beilstein. J. Org. Chem. 2022, 18, 1617–1624.
S. Moreno, L. Flemmich, R. Micura*; 'Synthesis of N4-acetylated 3-methylcytidine phosphoramidites for RNA solid-phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2022, 153, 285–291.
L. Bessler, N. Kaur, L.-M- Vogt, L. Flemmich, C. Siebenaller, M.-L. Winz, F. Tuorto, R. Micura, A. Ehrenhofer-Murray,* M. Helm*; 'Functional integration of a semi-synthetic azido-queuosine derivative into translation and a tRNA modification circuit', Nucleic Acids Res. 2022, 50, 10785–10800.
F. Erhard*, E. Saliba, A. Lusser, C. Toussaint, T. Hennig, B. Prusty, D. Kirschenbaum, K. Abadie, E. Miska, C. Friedel, I. Amit, R. Micura, L. Dölken*; 'Time-resolved single-cell RNA-seq using metabolic RNA labeling', Nat. Rev. Methods Primers 2022, 2, 77.
M. Mitter*, Z. Takacs, T. Köcher, R. Micura, C. Langer, D. Gerlich*; 'Sister-chromatid-sensitive Hi-C to map the conformation of replicated genomes'; Nat. Protoc. 2022, 17, 1486–1517.

2021

L. Flemmich, S. Heel, S. Moreno, K. Breuker, R. Micura*; 'A natural riboswitch scaffold with self-methylation activity'; Nat. Commun. 2021, 12, 3877.
L. Flemmich, S. Moreno, R. Micura*; 'Synthesis of O6-alkylated preQ1 derivatives'; Beilstein J. Org. Chem. 2021, 17, 2295–2301.
R. Bereiter #, M. Himmelstoß #, E. Renard, E. Mairhofer, M. Egger, K. Breuker, C. Kreutz, E. Ennifar, R. Micura*; 'Impact of 3-deazapurine nucleobases on RNA properties'; Nucleic Acids Res. 2021, 49, 4281–4293. # these authors contributed equally
X. Xu #, M. Egger #, H. Chen #, K. Bartosik, R. Micura*, A. Ren*; 'Insights into xanthine riboswitch structure and metal ion-mediated ligand recognition'; Nucleic Acids Res. 2021, 49, 7139 - 7153. # these authors contributed equally
O. A. Krasheninina, J. Thaler, M. E. Erlacher, R. Micura*; 'Amine-to-azide conversion on native RNA via metal-free diazo transfer opens new avenues for RNA manipulations'; Angew. Chem. Int. Ed. 2021, 60, 6970-6974.
C. Falschlunger, J. Leiter, R. Micura*; 'Elucidation of Ribozyme Mechanisms at the Example of the Pistol Ribozyme', in Ribozymes - Principles, Methods, Applications, Eds.: S. Müller, B. Masquida, W. Winkler, Wiley-VCH, Weinheim, 2021.

2020

R. Micura,* C. Höbartner*; 'Fundamental studies of functional nucleic acids: aptamers, riboswitches, ribozymes and DNAzymes'; Chem. Soc. Rev. 2020, 7331-7535.
M. Himmelstoß, K. Erharter, E. Renard, E. Ennifar,* C. Kreutz,* R. Micura*; '2′-O-Trifluoromethylated RNA – A powerful modification for RNA chemistry and NMR spectroscopy'; Chem. Sci. 2020, 11, 11322-11330.
C. Gasser #, I. Delazer #, E. Neuner +, K. Pascher +, K. Brillet, S. Klotz, L. Trixl, M. Himmelstoß, E. Ennifar, D. Rieder,* A. Lusser,* R. Micura*; 'Thioguanosine conversion enables mRNA life-time evaluation by RNA sequencing via double metabolic labeling (TUC-seq DUAL)'; Angew. Chem. Int. Ed. 2020, 59, 6881-6886. #,+ these authors contributed equally
M. Teplova #, C. Falschlunger #, O. Krasheninina #, M. Egger, A. Ren, D. J. Patel,* R. Micura*; 'On crucial roles of two hydrated Mg2+ ions in reaction catalysis of the pistol ribozyme'; Angew. Chem. Int. Ed. 2020, 59, 2837-2843. # these authors contributed equally
H. Chen #, M. Egger #, X. Xu #, L. Flemmich, O. Krasheninina, A. Sun, R. Micura,* A. Ren*; 'Structural distinctions between NAD+ riboswitch domains 1 and 2 determine differential folding and ligand binding'; Nucleic Acids Res. 2020, 48, 12394–12406. # these authors contributed equally
M. Mitter,* C. Gasser, Z. Takacs, C. H. C. Langer, W. Tang, G. Jessberger, C. T. Beales, E. Neuner, S. L. Ameres, J.-M. Peters, A. Goloborodko, R. Micura, D. W. Gerlich*; 'Conformation of sister-chromatids in the replicated human genome'; Nature 2020, 586, 139-144.
J. Leiter, D. Reichert, A. Rentmeister, R. Micura*; 'Practical Synthesis of Cap-4 RNA'; ChemBioChem 2020, 21, 265-271.
A. Lusser*, C. Gasser, L. Trixl, P. Piatti, D. Rieder, J. Bashin, I. Delazer, C. Riml, T. Amort, R. Micura*; 'Thiouridine-to-Cytidine conversion sequencing (TUC-seq) to measure mRNA transcription and degradation rates'; Meth. Mol. Biol. 2020, 2062, 191-211.
S. Werner, L. Schmidt, V. Marchand, T. Kemmer, C. Falschlunger, M. V. Sednev, G. Bec, E. Ennifar, C. Höbartner, R. Micura, Y. Motorin, A. Hildebrandt, M. Helm*; Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited transcriptomes'; Nucleic Acids Res. 2020, 48, 3734-3746.

2019

A. Sun #, C. Gasser #, F. Li #, H. Chen, S. Mair, O. Krasheninina, R. Micura,* A. Ren*; 'SAM-VI riboswitch structure and signature for ligand discrimination'; Nat. Commun. 2019, 10, 5728. # these authors contributed equally
E. Mairhofer, L. Flemmich, C. Kreutz, R. Micura*; 'Access to 3-Deazaguanosine Building Blocks for RNA Solid-Phase Synthesis Involving Hartwig-Buchwald C-N Cross-Coupling'; Org. Lett., 2019, 21, 3900-3903.
L. Zheng #, C. Falschlunger #, K. Huang, E. Mairhofer, S. Yuan, J. Wang, Dinshaw J. Patel*, R. Micura*, A. Ren*; 'Hatchet ribozyme structure and implications for cleavage mechanism'; Proc. Nat. Acad. Sci. 2019, 116, 10783-10791. # these authors contributed equally
S.V. Melnikov, N.F. Khabibullina, E. Mairhofer, O. Vargas-Rodriguez, N.M. Reynolds, R. Micura,* D. Söll*, Y.S. Polikanov*; 'Mechanistic insights into the slow peptide bond formation with D-amino acids in the ribosomal active site'; Nucl. Acids Res. 2019, 47, 2089-2100.
E. Fuchs, C. Falschlunger, R. Micura, K. Breuker*; 'The effect of adenine protonation on RNA phosphodiester backbone bond cleavage elucidated by deaza-nucleobase modifications and mass spectrometry'; Nucl. Acids Res. 2019, 47, 7223-7234.
C. Falschlunger, R. Micura*; 'Efficient access to N‐trifluoroacetylated 2′‐amino‐2′‐deoxyadenosine phosphoramidite for RNA solid‐phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2019, 150, 795-800.
E. Neuner, R. Micura*; 'Practical synthesis N-(di-n-butylamino)methylene protected 2-aminopurine riboside phosphoramidite for RNA solid-phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2019, 1941–1946.
C. Dégut, V. Schwarz, L. Ponchon, P. Barraud, R. Micura, C. Tisné*; 'Design of cross-linked RNA/protein complexes for structural studies'; Biochimie, 2019, 164, 95-98.
S. Mukherjee, L. Błaszczyk, W. Rypniewski, C. Falschlunger, R. Micura, A. Murata, C. Dohno, K. Nakatani*, A. Kiliszek*; 'Structural insights into synthetic ligands targeting A-A pairs in disease-related CAG RNA repeats'; Nucl. Acids Res. 2019, 47, 10906-10913.

2018

E. Neuner #, M. Frener #, A. Lusser*, R. Micura*; 'Superior cellular activities of azido- over amino-functionalized ligands for engineered preQ₁ riboswitches in E.coli'; RNA Biol. 2018, 15, 1376-1383. # these authors contributed equally
C. Gasser, J. Gebetsberger*, M. Gebetsberger, R. Micura*; 'SHAPE probing pictures Mg2+ dependent folding of small self-cleaving ribozymes'; Nucl. Acids Res. 2018, 46, 6983-6995.
T.P. Hoernes, K. Faserl, M.A. Juen, J. Kremser, C. Gasser, E. Fuchs, Xi. Shi, A. Siewert, H. Lindner, C. Kreutz, R. Micura, S. Joseph, C. Höbartner, E. Westhof, A. Hüttenhofer, M.D. Erlacher*; 'Translation of non-standard nucleotides reveals minimal requirements for codon-anticodon interactions', Nat. Commun. 2018, 9, 4865.

2017

S. Neuner #, C. Falschlunger #, E. Fuchs, M. Himmelstoss, A. Ren, D. J. Patel, R. Micura*; 'Atom-specific mutagenesis reveals structural and catalytic roles for active site–adenosine and Mg2+ in pistol ribozyme'; Angew. Chem. Int. Ed. 2017, 56, 16170-16174. # these authors contributed equally
C. Riml #, T. Amort #, D. Rieder #, C. Gasser, A. Lusser*, R. Micura*; 'Osmium-mediated 4sU-to-C transformation as key to study RNA dynamics by sequencing (TUC-seq)'; Angew. Chem. Int. Ed. 2017, 56, 13479-13483. # these authors contributed equally; see comment: 'RNA dynamics revealed by metabolic RNA labeling and biochemical nucleoside conversions. M. A. P. Baptista, L. Dölken. Nat. Methods 2018,15, 171-172.
N. Vusurovic #, R. B. Altman #, D. S. Terry, R. Micura*, S. C. Blanchard*; 'Pseudoknot formation seeds the twister ribozyme cleavage reaction coordinate'; J. Am. Chem. Soc. 2017, 139, 8186-8193. # these authors contributed equally
L. Zheng #, E. Mairhofer #, M. Teplova, J. Ma, D. J. Patel, R. Micura*, A. Ren*; 'Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme'; Nat. Commun. 2017, 8, 1180. # these authors contributed equally
A. Ren, R. Micura, D. J. Patel*, 'Structure-based mechanistic insights into catalysis of small self-cleaving ribozymes'; Curr. Opin. Chem. Biol. 2017, 41, 71-83.
C. Riml, A. Lusser, E. Ennifar, R. Micura*; 'Synthesis, thermodynamic properties, and crystal structure of RNA oligonucleotides containing 5-hydroxymethylcytosine'; J. Org. Chem. 2017, 82, 7939-7945.
H. Glasner, C. Riml, R. Micura, K. Breuker*; 'Label-free, direct localization and quantitation of the RNA nucleobase methylations m6A, m5C, m3U, and m5U by top-down mass spectrometry'; Nucl. Acids Res. 2017, 45, 8014-8025.
J. Gebetsberger, R. Micura*; 'Unwinding the twister ribozyme: From structure to mechanism'; WIREs RNA 2017, 8:e1402. ( Published Online: Nov 14 2016 DOI: 10.1002/wrna.1402 )
L. Jud, R. Micura*; 'An unconventional acid-labile nucleobase protection concept for guanosine phosphoramidites in RNA solid-phase synthesis'; Chem. Eur. J. 2017, 23, 3406–3413.
T. Amort, D. Rieder, A. Wille, D. Khokhlova-Cubberley, C. Riml, L. Trixl, X.-Y. Jia, R. Micura, A. Lusser*; 'Distinct 5-methylcytosine profiles in poly(A)RNA from mouse embryonic stem cells and the brain'; Genome Biology, 2017, 18, 1.
E. M. Danhart, M. Bakhtina, W.A. Cantara, A. B. Kuzmishin, X. Ma, B. L. Sanford, M. Kosutic, Y. Goto, H. Suga, K. Nakanishi, R. Micura, M. P. Foster*, K. Musier-Forsyth*; 'Conformational selection by a trans-acting editing domain'; Proc. Nat. Acad. Sci. 2017, 114, E6774-6783.
C. Riml, R. Micura; 'Automated chemical solid-phase synthesis and deprotection of 5-hydroxymethylcytosine-containing RNA'; in RNA Methylation (Eds. A. Lusser), Meth. Mol. Biol. 2017, 1562, 295-302.
S. Neuner, C. Kreutz*, R. Micura*; 'The synthesis of 15N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis'; Monatshefte für Chemie - Chemical Monthly, 2017, 148, 149-155. Special Issue "Organic chemistry – Austrian Chemical Society (GÖCH) work group"

2016

M. Frener, R. Micura*; 'Conformational rearrangements of individual nucleotides during RNA-ligand binding are rate-differentiated'; J. Am. Chem. Soc. 2016, 138, 3627-3630.
E. Mairhofer #, E. Fuchs #, R. Micura*; 'Facile synthesis of 3-deazaadenosine phosphoramidite for RNA solid-phase synthesis'; Beilstein J. Org. Chem. 2016, 12, 2556-2562. # these authors contributed equally
A. Ren #, N. Vusurovic #, J. Gebetsberger, P. Gao, M. Juen, C. Kreutz, R. Micura,* D.J. Patel*; 'Pistol ribozyme adopts a pseudoknot fold facilitating site-specific in-line cleavage'; Nat. Chem. Biol. 2016, 12, 702-708. # these authors contributed equally
S. Melnikov, J. Mailliot, L. Rigger, S. Neuner, B.-S. Shin, G. Yusupova, T. E. Dever,* R. Micura*, M. Yusupov*; 'Molecular insights into protein synthesis with proline residues'; EMBO Reports 2016, 17, 1776-1784.
S. Sothiselvam, S. Neuner, L. Rigger, D. Klepacki, R. Micura, N. Vázquez-Laslop,* A. S. Mankin*; 'The ribosome corrupted by macrolide antibiotics discriminates against specific substrates based on their charge and size'; Cell Reports 2016, 16, 1789-1799.
C. Riml, R. Micura*; 'Synthesis of 5-hydroxymethylcytidine and 5-hydroxymethyluridine-modified RNA'; Synthesis 2016, 48, 1108-1116.
S. Melnikov, J. Mailliot, B.-S. Shin, L. Rigger, G. Yusupova, R. Micura*, T. E. Dever,* M. Yusupov*; 'Crystal structure of hypusine-containing translation fachtor eIF5A bound to a rotated eukaryotic ribosome'; J. Mol. Biol. 2016, 428, 3570-3576.
S. Flür, R. Micura*; 'Chemical synthesis of RNA with site-specific methylphosphonate modifications'; Methods 2016, 107, 79-88.

2015

M. Kosutic #, S. Neuner #, A. Ren, S. Flür, C. Wunderlich, E. Mairhofer, N. Vusurovic, J. Seikowski, K. Breuker, C. Höbartner, D.J. Patel,* C. Kreutz,* R. Micura*; 'A Mini-Twister Variant and Impact of Residues/Cations on the Phosphodiester Cleavage of this Ribozyme Class'; Angew. Chem. Int. Ed. 2015, 54, 15128-15233. # these authors contributed equally
S. Neuner #, T. Santner #, C. Kreutz, R. Micura*; 'The "speedy" synthesis of atom-specific 15N imino/amido-labeled RNA'; Chem. Eur. J. 2015, 21, 11634-11643. # these authors contributed equally.
L. Jud #, M. Kosutic #, V. Schwarz, M. Hartl, C. Kreutz, K. Bister, R. Micura*; 'Expanding the scope of 2'-SCF3 modified RNA'; Chem. Eur. J. 2015, 21, 10400-10407. # these authors contributed equally.
M. Koch #, S. Flür #, C. Kreutz, R. Micura*, N. Polacek*; 'The role of a ribosomal RNA phosphate oxygen during the EF-G-triggered GTP hydrolysis'; Proc. Nat. Acad. Sci. 2015, 112, E6256–E6259. # these authors contributed equally.
C. Riml, H. Glasner, M. T. Rodgers, R. Micura, K. Breuker*; 'On the mechanism of RNA phosphodiester backbone cleavage in the absence of solvent'; Nucl. Acids Res. 2015, 43, 5171-5181.
T. Moschen, C. H. Wunderlich, R. Spitzer, J. Levic, R. Micura, M. Tollinger*, C. Kreutz*; 'Ligand-Detected Relaxation Dispersion NMR Spectroscopy: Dynamics of preQ1–RNA Binding'; Angew. Chem. Int. Ed. 2015, 570-573.
A.-S. Geiermann, R. Micura; 'Native chemical Ligation of hydrolysis-resistant 3'-NH-cystein-modified RNA'; Curr. Protoc. Nucl. Acid Chem. 2015, Wiley, 62:4.64.1-4.64.36.

2014

A. Ren #, M. Kosutic #, K. R. Rajashankar, M. Frener, T. Santner, E. Westhof, R. Micura*, D. J. Patel*; 'In-line alignment and Mg2+ coordination at the cleavage site of the env22 twister ribozyme'; Nat. Commun. 2014, 5: 5534. # these authors contributed equally; Pascal Legault, Faculty of 1000 Structural Biology.
S. Neuner, R. Micura*; 'Synthesis of aminoacetylated N6,N6-dimethyladenosine solid support for efficient access to hydrolysis-resistant 3'-charged tRNA mimics'; Bioorg. Med. Chem. 2014, 22, 6989-6995.
F. Spenkuch, G. Hinze, S. Kellner, C. Kreutz, R. Micura, T. Basché, M. Helm*; 'Dye label interference with RNA modification reveals 5-fluorouridine as non-covalent inhibitor'; Nucl. Acids Res. 2014, 42, 12735-12745.
J. Levic, R. Micura*; 'Syntheses of 15N-labeled pre-queuosine base derivatives'; Beilstein J. Org. Chem. 2014, 10, 1914-1918.
M. Kosutic #, L. Jud #, C. Da Veiga, M. Frener, K. Fauster, C. Kreutz, E. Ennifar*, R. Micura*; 'Surprising base pairing and structural properties of 2'-Trifluoromethylthio-Modified RNA'; J. Am. Chem. Soc. 2014, 136, 6656-6663. # these authors contributed equally
T. Santner, M. Hartl, K. Bister, R. Micura*; 'Efficient access to 3'-terminal azide-modified RNA for inverse Click-labeling patterns'; Bioconj. Chem. 2014, 25, 188-195 .
M. F. Soulière*, R. Micura*; 'Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies'; in Therapeutic applications of ribozymes and riboswitches (Eds. D. Lafontaine, A. Dubé), Meth. Mol. Biol. 2014, 1103, 227-239.

2013

M. F. Soulière, R. B. Altman, V. Schwarz, A. Haller, S. C. Blanchard*, R. Micura*; 'Tuning a riboswitch response through structural extension of a pseudoknot'; Proc. Nat. Acad. Sci. 2013, 110, E3256-E3264.
R. Micura*; 'RNA biophysics: A three-state balancing act'; Nature 2013, 499, 289-290 (News&Views).
L. Rigger, R. L. Schmidt, K. M. Holman, M. Simonovic*, R. Micura*; 'The synthesis of methylated, phosphorylated, and phosphonated 3'-aminoacyl-tRNASec mimics'; Chem. Eur. J. 2013, 19, 15872-15878. (Front cover)
M. Duval, A Korepanov, O. Fuchsbauer, P. Fechter, A. Haller, A. Fabbretti, L. Choulier, R. Micura, B. Klaholz, P. Romby*, M. Springer, S. Marzi*; 'Escherichia coli ribosomal protein S1 unfolds structured mRNA onto the ribosome for active translation initiation'; PLoS Biology 2013, 11(12), e1001731.
G. Bec, B. Meyer, M.-A. Gerard, J. Steger, K. Fauster, P. Wolff, D. Bernouf, R. Micura, P. Dumas, E. Ennifar*; 'Thermodynamics of HIV-1 reverse transcriptase in action elucidates the mechanism of action of non-nucleoside inhibitors'; J. Am. Chem. Soc. 2013, 135, 9743–9752.
R. Micura*, C. Kreutz*, K. Breuker*; 'A personal perspective on chemistry-driven RNA research'; Biopolymers 2013, 99, 1114-1123.
T. Amort, M. F. Soulière, A. Wille, X.-Y. Jia, H. Fiegl, H. Wörle, R. Micura, A. Lusser*; 'Long non-coding RNAs as targets for cytosine methylation'; RNA Biology 2013, 10, 1002-1008 .
A. Haller, R. B. Altman, M. Soulière, S. C. Blanchard*, R. Micura*; 'Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution'; Proc. Nat. Acad. Sci. 2013, 110, 4188-4193.
M. F. Soulière, A. Haller, T. Santner, R. Micura*; 'New Insights into Gene Regulation - High Resolution Structures of Cobalamin Riboswitches'; Angew. Chem. Int. Ed. 2013, 52, 1874-1877; Angew. Chem. 2013, 125, 1924-1927.

2012

K. Fauster, C. Kreutz, R. Micura*; '2'-SCF3 uridine - A novel powerful label for probing RNA structure and function by 19F-NMR spectroscopy'; Angew. Chem. Int. Ed. 2012, 51, 13080-13084; Angew. Chem. 2012, 124,13257-13261
V. Siegmund, T. Santner, R. Micura, A. Marx*; 'Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides'; Chem. Comm. 2012, 48, 9870-9872
T. Santner #, U. Rieder #, C. Kreutz*, R. Micura*; 'Pseudoknot preorganization of the preQ1 class I riboswitch'; J. Am. Chem. Soc. 2012, 134, 11928-11931. # these authors contributed equally
A.-S. Geiermann, R. Micura*; 'Selective desulfurization significantly expands sequence variety of 3’-peptidyl-tRNA mimics obtained by native chemical ligation'; Chembiochem 2012, 12,1742-1745
T. Santner, V. Siegmund, A. Marx, R. Micura*; The synthesis of 2'-methylseleno adenosine and guanosine 5'-triphosphates; Bioorg. Med. Chem. 2012, 20, 2416-2418. (Front cover)
K. Fauster, M. Hartl, T. Santner, M. Aigner, C. Kreutz, K. Bister*, E. Ennifar*, R. Micura*; 2-Azido RNA, a Versatile Tool for Chemical Biology: Synthesis, X-ray Structure, siRNA Applications, Click Labeling; ACS Chem. Biol. 2012, 7, 581-589. (Front cover)
R. Micura; book review on 'Metal Ions in Life Sciences, Vol. 9., Structural and Catalytic Roles of Metal Ions in RNA', A. Sigel, H. Sigel, R.K.O. Sigel (Eds.) RSC Publishing (2011); Coord. Chem. Rev. 2012, 256, 370
D. Graber, K. Trappl, J. Steger, A.-S. Geiermann, L. Rigger, H. Moroder, N. Polacek, R. Micura*; 'Deoxyribozyme-based, semisynthetic access to stable peptidyl-tRNAs exemplified by tRNA(Val) carrying a macrolide antibiotic resistance peptide'; in Ribozymes (Ed. J. S. Hartig), Meth. Mol. Biol. 2012, 848, 201-213.

2011

A.-S. Geiermann, N. Polacek, R. Micura*; 'Native chemical ligation of hydrolysis-resistant 3’-peptidyl-tRNA mimics'; J. Am. Chem. Soc. 2011, 133, 19068-19071.
M. Soulière, A. Haller, R. Rieder, R. Micura*; 'A powerful approach for the selection of 2-aminopurine substitution sites to investigate RNA folding'; J. Am. Chem. Soc. 2011, 133, 16161-16167
V. Siegmund, T. Santner, R. Micura*, A. Marx*; 'Enzymatic synthesis of 2'-methylseleno-modified RNA'; Chem. Sci. 2011, 2, 2224-2231
J. Steger, R. Micura*; 'Functionalized polystyrene supports for solid-phase synthesis of glycyl-, alanyl-, and isoleucyl-RNA conjugates as hydrolysis-resistant mimics of peptidyl-tRNAs'; Bioorg. Med. Chem. 2011, 19, 5167-5174
A. Haller #, M. F. Soulière #, R. Micura*; 'The dynamic nature of RNA as key to understanding riboswitch mechanisms'; Acc. Chem. Res. 2011, 44, 1339-1348. # these authors contributed equally
A. Haller, U. Rieder, M. Aigner, S. C. Blanchard*, R. Micura*; 'Conformational capture of the SAM-II riboswitch'; Nat. Chem. Biol. 2011, 7, 393-400.
News&Views by Ming C. Hammond; 'RNA folding: A tale of two riboswitches'; Nat. Chem. Biol. 2011, 7, 342-343
H. Ramu, N. Vázquez-Laslop, D. Klepacki, Q. Dai, J. Piccirilli, R. Micura, A. S. Mankin*; 'Nascent Peptide in the Ribosome Exit Tunnel Affects Functional Properties of the A-Site of the Peptidyl Transferase Center'; Mol. Cell. 2011, 41, 321-330.
Preview by D. N. Wilson*; 'Peptides in the Ribosomal Tunnel Talk Back'; Mol. Cell 2011, 41, 247-248
M. Aigner, M. Hartl, K. Fauster, J. Steger, K. Bister, R. Micura*; 'Chemical Synthesis of Site-Specifically 2'-Azido-Modified RNA and Potential Applications for Bioconjugation and RNA Interference'; Chembiochem 2011, 11, 47-51

2010

J. Steger, D. Graber, H. Moroder, A.-S. Geiermann, M. Aigner, R. Micura*; 'Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′-Azido-3′-Deoxyadenosine RNA'; Angew. Chem. Int. Ed. Engl. 2010, 49, 7470-7472; Angew. Chem. 2010, 122, 7 632-7634
Y. Wang, J. Ludwig, C. Schuberth, M. Goldeck, M. Schlee, H. Li, S. Juranek, G. Sheng, R. Micura, T. Tuschl, G. Hartmann, D. J. Patel; 'Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I'; Nat. Struct. Mol. Biol. 2010, 17, 781-787.
D. Graber, H. Moroder, J. Steger, K. Trappl, N. Polacek, R. Micura*; 'Efficient semisynthesis of hydrolysis-resistant 3'-peptidyl-tRNA conjugates containing genuine tRNA modifications'; Nucl. Acids Res. 2010, 38, 6796-6802.
U. Rieder, C. Kreutz, R. Micura*; 'Folding of a transcriptionally acting preQ1 riboswitch'; Proc. Natl. Acad. Sci. U.S.A. 2010, 107, 10804-10809.
N. Clementi, A. Chirkova, B. Puffer, R. Micura, N. Polacek*; 'Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation'; Nat. Chem. Biol. 2010, 6, 344-351.
A. Chirkova, M. Erlacher, R. Micura, N. Polacek*; 'Chemically engineered ribosomes: A new frontier in synthetic biology'; Curr. Org. Chem. 2010, 14, 148-161.

2009

A.-S. Geiermann, R. Micura*; 'Chasing after antibiotic leads'; Chem. Biol. 2009, 16, 1024-1025 (Preview).
B. Puffer, C. Kreutz, U. Rieder, M.-O. Ebert, R. Konrat, R Micura*; '5-Fluoro pyrimidines: Labels to probe DNA and RNA secondary structures by 1D 19F NMR spectroscopy'; Nucl. Acids Res. 2009, 37, 7728-7740.
K. De Mulder, D. Pfister, G. Kuales, B. Egger, W. Salvenmoser, M. Willems, J. Steger, K. Fauster, R. Micura, G. Borgonie, P. Ladurner*; 'Stem cells are differentially regulated during development, regeneration and homeostasis in flatworms'; Dev. Biol. 2009, 334, 198-212.
U. Rieder, K. Lang, C. Kreutz, N. Polacek, R. Micura*; 'Evidence for Pseudoknot Formation of Class I preQ1 Riboswitch Aptamers'; Chembiochem 2009, 10, 1141-1144.
H. Moroder #, J. Steger #, D. Graber, K. Fauster, K. Trappl, V. Marquez, N. Polacek, D. N. Wilson, R. Micura*; 'Nonhydrolyzable RNA–Peptide Conjugates: A Powerful Advance in the Synthesis of Mimics for 3'-Peptidyl tRNA Termini'; Angew. Chem. Int. Ed. Engl. 2009, 48, 4056-4060; Angew. Chem. 2009, 121, 4116-4120. # these authors contributed equally
V. Olieric #, U. Rieder #, K. Lang, A. Serganov, C. Schulze-Briese, R. Micura, P. Dumas, E. Ennifar*; 'A fast strategy for selenium derivatization directed to phasing of X-ray RNA structures'; RNA 2009, 15, 707-715.# these authors contributed equally
R. Rieder, C. Höbartner, R. Micura*; 'Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications', in Riboswitches (Ed. A. Serganov), Meth. Mol. Biol. 2009, 540, 15-24.

2008

D. Graber, H. Moroder, R. Micura*; '19F NMR spectroscopy for the analysis of RNA secondary structure populations'; J. Am. Chem. Soc. 2008, 130, 17230–17231.
P. S. Pallan, C. Kreutz, S. Bosio, R. Micura*, Martin Egli*; 'Effects of N2,N2-dimethylguanosine on RNA structure and stability: crystal structure of an RNA duplex with tandem m22G:A pairs', RNA 2008, 14, 2125-2135.
K. Lang, R. Micura*; 'Preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments', Nature Protocols 2008, 3, 1457-1466.
K. Lang, M. Erlacher, D. N. Wilson, R. Micura*, N. Polacek*; 'The role of 23S rRNA residue A2451 in ribosomal peptide bond synthesis revealed by atomic mutagenesis'; Chem. Biol. 2008, 15, 485-492.
Preview by M. Pech, K. H. Nierhaus*; 'Ribosomal peptide-bond formation'; Chem. Biol. 2008, 15, 417-419.
S. Freisz, K. Lang, R. Micura, P. Dumas, E. Ennifar*; 'Binding of aminoglycoside antibiotics to the duplex form of the HIV-1 genomic RNA dimerization initiation site'; Angew. Chem. 2008, 120, 4178-4181; Angew. Chem. Int. Ed. Engl. 2008, 47, 4110-4113.
B. Puffer, H. Moroder, M. Aigner, R. Micura*; '2'-Methylseleno modified oligoribonucleotides for X-ray crystallography synthesized by the ACE RNA solid-phase approach'; Nucl. Acids Res. 2008, 36, 970-983.
C. Kreutz, R. Micura*; 'Investigations on fluorine-labeled ribonucleic acids by 19F NMR spectroscopy'; Modified Nucleotides in Biochemistry, Biotechnology and Medicine; Ed.: P. Herdewijn, Wiley-VCH, 2008, 3-22.
R. Micura; contributions to 'Nucleic Acids from A to Z – A Concise Encyclopedia'; Ed.: Sabine Müller, Wiley-VCH, 2008.

2007

F. Li, P. Pallan, M. Maier, K. Rajeev, S. Mathieu, C. Kreutz, Y. Fan, J. Sanghvi, R. Micura, E. Rozners, M. Manoharan, M. Egli*; 'Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme'; Nucl. Acids Res. 2007, 35, 6424-6438.
K. Lang, R. Rieder, R. Micura*; 'Ligand-induced folding of the thiM TPP riboswitch investigated by a structure-based fluorescence spectroscopic approach'; Nucl. Acids Res. 2007, 35, 5370-5378.
M. Amort, B. Wotzel, K. Bakowska-Zywicka, M. D. Erlacher, R. Micura, and N. Polacek*; 'An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation termination'; Nucl. Acids Res. 2007, 35, 5130-5140.
R. Rieder, K. Lang, D. Graber, R. Micura*; 'Ligand-Induced Folding of the Adenosine Deaminase A-Riboswitch and Implications on Riboswitch Translational Control'; Chembiochem 2007, 8, 896 - 902.
R. Micura; 'RNA – Struktur und Funktion'; Trendbericht, Nachrichten aus der Chemie 2007, 55, 279-284.

2006

R. Micura, C. Höbartner, R. Rieder, C. Kreutz, B. Puffer, K. Lang, H. Moroder; 'Preparation of 2'-Deoxy-2'-Methylseleno-Modified RNA', Curr. Protoc. Nucl. Acid Chem. 2006, Wiley, C1.15.1. - C1.15.34.
H. Moroder, C. Kreutz, K. Lang, A. Serganov*, and R. Micura*; 'Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs'; J. Am. Chem. Soc. 2006, 128, 9909-9918.
C. Kreutz, H. Kählig, R. Konrat*, R. Micura*; 'A General Approach for the Identification of Site-Specific RNA Binders by 19F-NMR Spectroscopy - Proof of Concept'; Angew. Chem. 2006, 118, 3528-3532; Angew. Chem. Int. Ed. Engl. 2006, 45, 3450-3453.
M. D. Erlacher #, K. Lang #, B. Wotzel, R. Rieder, R. Micura*, N. Polacek*; 'Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2'-OH at A2451 of 23S rRNA'; J. Am. Chem. Soc. 2006, 128, 4453-4459. # these authors contributed equally
R. Micura*; 'Programmable ligand-controlled riboregulators'; Angew. Chem. 2006, 118, 32-34; Angew. Chem. Int. Ed. Engl. 2006, 45, 30-31.

2005

C. Kreutz, H. Kählig, R. Konrat*, R. Micura*, 'Ribose 2'-F labeling: A simple tool for the characterization of RNA secondary structure equilibria by 19F-NMR spectroscopy', J. Am. Chem. Soc. 2005, 127, 11558-11559.
C. Höbartner, R. Rieder, C. Kreutz, B. Puffer, K. Lang, A. Polonskaia, A. Serganov, R. Micura*, 'Syntheses of RNAs with up to 100 nucleotides containing site-specific 2'-Se-methyl labels for use in X-ray crystallography', J. Am. Chem. Soc. 2005, 127, 12035-12045.
M. D. Erlacher, K. Lang, N. Shankaran, B. Wotzel, A. Hüttenhofer, R. Micura, A. S. Mankin, N. Polacek*; 'Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-O-hydroxyl group of A2451'; Nucl. Acids Res. 2005, 33, 1618-1627.
A. Serganov, S. Keiper, L. Malinina, V. Tereshko, E. Skripkin, C. Hobartner, A. Polonskaia, A. T. Phan, R. Wombacher, R. Micura, Z. Dauter, A. Jaschke*, D. J. Patel*; 'Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation'; Nat. Struct. Mol. Biol. 2005, 12, 218 - 224.

2004

A. Serganov, Y. R. Yuan, O. Pikovskaya, A. Polonskaia, L. Malinina, A.T. Phan, C. Höbartner, R. Micura, R.R. Breaker, D.J. Patel*; 'Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs', Chem. Biol. 2004, 11, 1729-1741.
R. Micura*; 'Genetic control by a natural metabolite-responsive ribozyme'; Angew. Chem. 2004, 116, 4797-4799; Angew. Chem. Int. Ed. Engl. 2004, 43, 4692-4694.
C. Hoebartner, H. Mittendorfer, K. Breuker, R. Micura*; 'Triggering of RNA secondary structures by a functionalized nucleobase'; Angew. Chem. 2004, 116, 4012-4015; Angew. Chem. Int. Ed. Engl. 2004, 43, 3922-3925.
C. Hoebartner, R. Micura*; 'The chemical synthesis of selenium-modified oligoribonucleotides and their enzymatic ligation leading to an U6 snRNA stem-loop segment'; J. Am. Chem. Soc. 2004, 126, 1141-1149.
R. Micura*, C. Hoebartner; 'Equilibria of RNA secondary structures', in Bioorganic Chemistry Highlights II: From Chemistry to Biology, Ed. C. Schmuck, H. Wennemers, WILEY-VCH, Weinheim, 2004, 3-16.

2003

S. Pitsch, S. Wendeborn, R. Krishnamurthy*, A. Holzner, M. Minton, M. Bolli, C. Miculka, N. Windhab, R. Micura, M. Stanek, B. Jaun, A. Eschenmoser*; 'The ß-D-Ribopyranosyl-(4'-2')-Oligonucleotide System ('Pyranosyl-RNA'): Synthesis and Resume of Base-Pairing Properties'; Helv. Chim. Acta 2003, 12, 4270-4363.
R. Micura*, and C. Hoebartner; 'On RNA secondary structure rearrangements and equilibria of small RNAs'; ChemBioChem 2003, 4, 984-990.
C. Hoebartner, C. Kreutz, E. Flecker, E. Ottenschlaeger, W. Pils, K. Grubmayr, R. Micura*; 'The synthesis of 2'-O-[(triisopropylsilyl)oxy]methyl (TOM) phosphoramidites of methylated ribonucleosides (m1G, m2G, m22G, m1I, m3U, m4C, m6A, m62A) for use in automated RNA solid-phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2003, 134, 851-873.
C. Hoebartner, R. Micura*; 'Bistable secondary structures of small RNAs and their structural probing by comparative imino proton NMR spectroscopy'; J. Mol. Biol. 2003, 325, 421-431.

2002

R. Micura*; 'Small interfering RNAs and their chemical synthesis'; Angew. Chem. 2002, 114 (13), 2369-2373; Angew.Chem. Int. Ed. Engl. 2002, 41 (13), 2265-2269.
C. Hoebartner, M.-O. Ebert, B. Jaun, R. Micura*; 'RNA two-state conformation equilibria and the effect of nucleobase methylation'; Angew. Chem. 2002, 114 (4), 619-623; Angew. Chem. Int. Ed. Engl. 2002, 41 (4), 605-609.

2001

R. Micura*, W. Pils, C. Hoebartner, K. Grubmayr, M.-O. Ebert, B. Jaun; 'Methylation of nucleobases in RNA oligonucleotides mediates hairpin-duplex conversion'; Nucleic Acids Res. 2001, 29, 3997-4005.
R. Micura*, W. Pils, K. Grubmayr, M.-O. Ebert, B. Jaun; 'Bridged Cyclic Oligoribonucleotides - towards Models for Codon-Anticodon Pairing'; Nucleosides, Nucleotides, Nucleic Acids 2001, 20, 1287-1289.

2000

R. Micura*, W. Pils, and K. Grubmayr; 'Bridged Cyclic Oligoribonucleotides as Model Compounds for Codon-Anticodon Pairing'; Angew. Chem. 2000, 112, 956-958; Angew. Chem. Int. Ed. Engl. 2000, 39, 922-925.
W. Pils and R. Micura*, 'Flexible non-nucleotide linkers as loop replacements in short duplex RNAs'; Nucleic Acids Res. 2000, 28, 1859-1863.
M.- O. Ebert, Bernhard Jaun* and W. Pils, R. Micura; 'On RNA triplet interactions: NMR study of the short intramolecular duplex formed by (D)-r[GCAm1G-p-O(CH2CH2O)6-p-UGCC]'; Helv. Chem. Acta 2000, 83, 2338-2343.

1999

R. Micura*; 'Cyclic Oligoribonucleotides (RNA) by Solid Phase Synthesis'; Chemistry Eur. J. 1999, 5, 2077-2082.
R. Micura, R. Kudick and A. Eschenmoser*; 'Opposite Orientation of Backbone Inclination in pyranosyl-RNA and homo-DNA Correlates with Opposite Directionality of Duplex Properties'; Angew. Chem. 1999, 111, 715-718; Angew. Chem. Int. Ed. Engl. 1999, 38, 680-683

1997

M. Bolli, R. Micura, S. Pitsch and A. Eschenmoser*; 'Pyranosyl-RNA: Further Observations on Replication'; Helv. Chem. Acta 1997, 80, 1901-1951.
M. Bolli, R. Micura, and A. Eschenmoser*; 'Pyranosyl-RNA: Chiroselective self-assembly of base sequences by ligative oligomerization of tetranucleotide-2',3'-cyclophosphates (with a commentary concerning the origin of biomolecular homochirality)'; Chem.&Biol. 1997, 4, 309-320.
R. Micura, M. Bolli, N. Windhab and A. Eschenmoser*; 'Pyranosyl-RNA Also Forms Hairpin Structures', Angew. Chem. 1997, 109, 899-902; Angew. Chem. Int. Ed. Engl. 1997, 36, 870-873.

1996

R. Micura* and K. Grubmayr; 'The Enantiomers of Phycocyanobilin Dimethyl Ester: A Facile Approach via Semipreparative Enantioselective Chromatography of their Ring-D Methyl Imino Esters'; Enantiomer 1996, 1, 109-113.
H. Falk*, R. Micura, M. Stanek, and R. Wutka; 'Structural Aspects of Native and Acid or Enzyme Degraded Amylopectins - a 13C NMR Study', Starch/Stärke 1996; 48, 344-346.
H. Falk*, Q.-Q. Chen, and R. Micura; 'On the Chemistry of Pyrrole Pigments, XCVII: Synthesis, Stereochemistry, and Solvatochromic Effects of a 1-(Dipyrrinon-9-yl)-3-(dipyrrinon-9-ylidene)-1-propene'; Monatsh. 1996, 127, 77-83.

1995

R. Micura and K. Grubmayr*; 'A Phycocyanobilin Seryliminoester as a New Model for the Chromophore-Protein Interaction in Phytochrome'; Angew. Chem. 1995, 107, 1896-1898; Angew. Chem. Int. Ed. Engl. 1995, 34, 1733-1735.
Q.-Q. Chen, H. Falk*, and R. Micura; 'On the Chemistry of Pyrrole Pigments, XCII: Syntheses of 1,2-Bis-pyrrolylethanes'; Monatsh. 1995, 126, 473-479.

1994

R. Micura and K. Grubmayr*; 'Long-wavelength absorbing derivatives of phycocyanobilin: new structural aspects on phytochrome'; Bioorg. Med. Chem. Lett. 1994, 4, 2517-2522.